3500 Genetic Analyzer is a fluorescence based 8-capillary sequencing instrument which is based on Sanger DNA sequencing. The system is configured with 50 cm array and an integrated software for instrument control, data collection, quality control, base-calling and size-calling of samples. We use BigDyeTM Terminator v3.1 cycle sequencing kit, POP-7TM, and BigDye XterminatorTM for short-to-long-read sequencing. The system has a varied range of applications like microbial sequencing, targeted DNA sequencing, epigenetic analysis, pathogen analysis, genetic disease research, SNP analysis and biomarker analysis. Currently, a range of cloned DNA, PCR products and BAC DNA are analyzed with a read length of up to 1000 bp.