Monolith NT.115 uses MicroScale Thermophoresis (MST) technology to quantify binding events. One partner is typically labeled with a fluorescent dye and becomes an extremely sensitive reporter for all binding interactions. Monolith is all about accurately determining binding affinity in minutes. The strength of the interactions between a fluorescently labeled or intrinsically fluorescent sample and a binding partner (or ligand) are measured while a temperature gradient is applied over time. These events, weak or strong, cause a change in MST signal that is easily detected by plotting the normalized fluorescence against concentration of ligand. The results are automatically translated into precise and quantitative Kd values. Interactions can be studied in immobilization-free format.